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FIB-SEM is also widely used for the three-dimensional reconstruction of samples, particularly in the field of biological imaging. By sequentially milling away thin layers of the sample and imaging each layer using the SEM, researchers can obtain a series of two-dimensional images that can be combined to create a three-dimensional representation of the sample.
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Despite its many advantages, FIB-SEM also has some limitations. This includes the potential for sample damage due to ion beam irradiation and the relatively slow milling and imaging process compared to other techniques.
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Scanning electron microscopy has made significant contributions to the field of biology and life sciences. This enables researchers to study the intricate details of cellular structures, tissues, and biological materials at high resolution.
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Magnification rein an SEM can Beryllium controlled over a range of about 6 orders of magnitude from about 10 to 3,000,000 times.[28] Unlike optical and transmission electron microscopes, image magnification in an SEM is not a function of the power of the objective lens. SEMs may have condenser and objective lenses, but their function is to focus the beam to a spot, and not to image the specimen. Provided the electron gun can generate a beam with a sufficiently small diameter, an SEM could in principle work entirely without condenser or objective lenses.
The detected signals from these detectors are processed and used to form images that reveal various aspects of the sample. Rein SEM, the image formation process involves scanning the electron beam across the sample surface hinein a raster pattern. This information is then used to generate a two-dimensional image, where each Bildpunkt corresponds to a specific location on the sample surface, and its intensity represents the signal strength at that point.
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